About Gradient Thermal Cycler
Polymerase Chain Reaction (PCR) is widely used in molecular biology. as PCR progresses, the DNA thus generated is itsel used as template for replication. With PCR it is possible to amplify a single or few copies of a specific DNA fragment across several order of magnitude, generating millions copies of the DNA fragment
Precision Thermal Control for Effective PCR ResultsWith a programmable gradient range of 1 to 25C across the block and an accuracy of 0.2C at 55C, this cycler ensures outstanding consistency and reproducibility in all your DNA or RNA amplification tasks. The advanced Peltier-based cooling and high thermal conductivity aluminum block deliver rapid, uniform temperature transitions, making it ideal for sensitive laboratory protocols.
Advanced Features for Modern LaboratoriesThis thermal cycler is designed with a digital control interface, large graphical LCD, and multilingual support, making it easy for users across different backgrounds. Automatic heated lid, end-of-run acoustic alarm, lid lock during the run, and quick-start function enhance safety and convenience. Internal memory, plus external USB ports, facilitate robust program storage and software updates.
Flexible and Reliable PCR AmplificationSuitable for standard PCR test strips and 96-well microplates, the cycler supports up to 96 samples per run with adjustable test speeds (1-3C/sec). Auto-recovery restores run programs after power interruptions, ensuring no precious experiments are lost. Its compact benchtop design and semi-automatic operation fit seamlessly into any lab environment.
FAQ's of Gradient Thermal Cycler:
Q: How do I update the software on the Gradient Thermal Cycler?
A: You can update the cycler's software via the built-in USB port. Simply download the latest software from the supplier's website, transfer it to a USB drive, and connect it to the cycler to follow on-screen instructions for a seamless update.
Q: What are the benefits of the automatic heated lid with a lid lock during runs?
A: The automatic heated lid ensures even temperature distribution across your samples by preventing condensation, while the lid lock improves safety during operation, reducing the risk of accidental exposure or temperature instability.
Q: When should I use the gradient function in a PCR application?
A: The gradient feature is best utilized during protocol optimization, such as determining the optimal annealing temperature for new primer sets, allowing you to test a range of temperatures in a single run.
Q: Where can the Gradient Thermal Cycler be used most effectively?
A: This equipment is ideal for molecular biology and clinical research laboratories requiring reliable PCR and DNA/RNA amplification, as its features support both routine and high-throughput sample processing.
Q: What is the process for program backup and recovery after a power loss?
A: The system includes an auto-recovery function, which saves your run progress. In the event of a power interruption, the cycler will automatically restore and continue your PCR program once power is reinstated.
Q: How does the cycler store and export PCR run data?
A: PCR run data are saved internally and can be transferred externally via USB storage. The USB and RS232 interfaces allow data download or connection to compatible laboratory management systems for easier record-keeping and analysis.